Services Price List
Primary Cell Lines Isolation/Reprogramming
The core uses RNA-based and episomal-based reprogramming technology to reprogram somatic cells into induced pluripotent stem cells (iPSCs).
| Service | Description | Price |
| Fibroblast Isolation | Includes isolation, expansion, and freezing of fibroblasts from skin biopsy (mycoplasma tested) | $740.03 |
| Fibroblast reprogramming | RNA‐based reprogramming Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested) | $3,359.49 |
| Renal epithelial cell (REC) isolation | Includes isolation, expansion, and freezing of RECs from urine sample (mycoplasma tested) | $885.86 |
| REC Reprogramming | RNA‐based reprogramming Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested) | $3,574.15 |
| Peripheral blood mononuclear cell (PBMC) isolation | Includes isolation and freezing of PBMCs (1‐2 vials) | $372.06 |
| PBMC Reprogramming | Episomal reprogramming Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested, loss of episomes confirmed by PCR) | $3,377.89 |
iPSC Characterization Services and Quality Control
The core provides services to analyze newly reprogrammed (or client provided) iPSC lines to provide a complete characterization profile and demonstrate pluripotency.
| Service | Description | Price |
| Thaw iPSCs | Thaw vial into two wells of a 6‐well plate | $181.22 |
| Expand and Freeze iPSCs | Freeze 8‐12 vials | $364.65 |
| Mycoplasma Testing | All samples are tested for mycoplasma contamination | $95.18 |
| Identity testing | STR analysis | $139.99 |
| Pluripotency Analysis | Immunofluorescence for TRA‐1‐60, NANOG, OCT 3/4 | $397.56 |
| qPCR for OCT4, NANOG, DNMT3B | $311.04 | |
| G‐Band Karyotyping | Chromosome analysis, optimized for human iPSCs | $736.23 |
| ddPCR (core operated) | Price per 24 samples | $344.58 |
Gene Editing
- Investigator can provide iPSCs or use a SCBDMC control iPSC line (+$498.88)
- Includes design of gene modification strategy and purchase of all reagents
- iPSCs are modified with Cas9 mRNA and synthesized gRNA
| Service | Description | Price |
| Gene knock‐out by INDEL | Modified iPSCs are clonally expanded and the INDEL mutation is confirmed by Sanger sequencing | $10,775.96 |
| SNP correction or introduction | Modified iPSCs are clonally expanded and the desired mutation is confirmed by Sanger sequencing Deliver 4‐6 frozen vials (identity and mycoplasma tested) | $14,073.09 |
| Targeted fluorescence knock‐in | Up to 24 genetically modified iPSC lines are clonally expanded Deliver 2 frozen vials (identity tested) IMPORTANT: We cannot functionally validate successful targeting for genes that require differentiation. Reporter validation and clonality must be confirmed by the investigator. | $17,816.79 |
| Constitutive fluorescence knock‐in | mTagBFP2, mWasabi, tdTomato, iRFP670 Most FPs can be localized to the nucleus (NLS) or membrane (CAAX) Clonally expanded iPSC line (3‐4 frozen vials, identity and mycoplasma tested) | $4,110.51 |
Modified mRNA
Subject to availability
| Service | Description | Price |
| Modified mRNA | In stock modified mRNAs (per µg); bulk discounts up to 30% | $12.66 |
| RNA Reprogramming Kit | Sufficient mRNA/miR cocktail and transfection buffer to reprogram a full 6‐well plate | $739.96 |
| Custom modified mRNA | Cloning cDNA template and mRNA synthesis | Inquire |
Biorepository
The core is equipped with a top of the line setup for cell cryopreservation and storage. Our Isothermal Liquid Nitrogen Freezers are safe; provide no cross-contamination and the best in the industry for temperature uniformity.
| Service | Description | Price |
| Vial Storage | Price per vial per month | $0.06 |
| Vial of wild‐type iPSCs | Derived from fibroblasts, reprogrammed with RNA | $498.88 |
| Vial of modified iPSCs | Constitutively fluorescent iPSCs (mWasabi; strong CAGG promoter, targeted to AAVS safe) Core generated genetically modified iPSCs | $848.46 |
Other Services
| Service | Description | Price |
| iPSC Training | Hands‐on training includes thawing, culture, passaging, and freezing of human iPSCs | $1,466.55 |
| Consulting | Example expertise includes but is not limited to: CRISPR design, custom assay design (eg ddPCR), viral vector design, custom mRNA production, training, etc) | $109.28/h |
Listed prices are for the researchers affiliated with the University of Colorado. External clients need to contact the core to inquire about external prices.
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