Services Price List
Primary Cell Lines Isolation/Reprogramming
The core uses RNA-based and episomal-based reprogramming technology to reprogram somatic cells into induced pluripotent stem cells (iPSCs).
| Service | Description | Price |
| Fibroblast Isolation | Includes isolation, expansion, and freezing of fibroblasts from skin biopsy (mycoplasma tested) | $1,013.41 |
| Fibroblast reprogramming | RNA‐based reprogramming Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested) | $4,517.77 |
| Renal epithelial cell (REC) isolation | Includes isolation, expansion, and freezing of RECs from urine sample (mycoplasma tested) | $1,006.38 |
| REC Reprogramming | RNA‐based reprogramming Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested) | $5,095.09 |
| Peripheral blood mononuclear cell (PBMC) isolation | Includes isolation and freezing of PBMCs (1‐2 vials) | $550.90 |
| PBMC Reprogramming | Episomal reprogramming Deliver 2‐4 clonally expanded iPSC lines (2‐4 frozen vials per iPSC line, identity and mycoplasma tested, loss of episomes confirmed by PCR) | $4,813.16 |
iPSC Characterization Services and Quality Control
The core provides services to analyze newly reprogrammed (or client provided) iPSC lines to provide a complete characterization profile and demonstrate pluripotency.
| Service | Description | Price |
| Thaw iPSCs | Thaw vial into two wells of a 6‐well plate | $264.90 |
| Expand and Freeze iPSCs | Freeze 8‐12 vials | $505.67 |
| Mycoplasma Testing | All samples are tested for mycoplasma contamination | $126.52 |
| Identity testing | STR analysis | $210.00 |
| Pluripotency Analysis | Immunofluorescence for TRA‐1‐60, NANOG, OCT 3/4 | $534.83 |
| qPCR for OCT4, NANOG, DNMT3B | $467.73 | |
| G‐Band Karyotyping | Chromosome analysis, optimized for human iPSCs | $837.45 |
| ddPCR (core operated) | Price per 24 samples | $441.87 |
Gene Editing
- Investigator can provide iPSCs or use a SCBDMC control iPSC line (+$760.03)
- Includes design of gene modification strategy and purchase of all reagents
- iPSCs are modified with Cas9 mRNA and synthesized gRNA
| Service | Description | Price |
| Gene knock‐out by INDEL | Modified iPSCs are clonally expanded and the INDEL mutation is confirmed by Sanger sequencing | $14,186.68 |
| SNP correction or introduction | Modified iPSCs are clonally expanded and the desired mutation is confirmed by Sanger sequencing Deliver 4‐6 frozen vials (identity and mycoplasma tested) | $18,409.78 |
| Targeted fluorescence knock‐in | Up to 24 genetically modified iPSC lines are clonally expanded Deliver 2 frozen vials (identity tested) IMPORTANT: We cannot functionally validate successful targeting for genes that require differentiation. Reporter validation and clonality must be confirmed by the investigator. | $21,435.38 |
| Constitutive fluorescence knock‐in | mTagBFP2, mWasabi, tdTomato, iRFP670 Most FPs can be localized to the nucleus (NLS) or membrane (CAAX) Clonally expanded iPSC line (3‐4 frozen vials, identity and mycoplasma tested) | $7,628.36 |
Modified mRNA
Subject to availability
| Service | Description | Price |
| Modified mRNA | In stock modified mRNAs (per µg); bulk discounts up to 30% | $16.83 |
| RNA Reprogramming Kit | Sufficient mRNA/miR cocktail and transfection buffer to reprogram a full 6‐well plate | $1,001.11 |
| Custom modified mRNA | Cloning cDNA template and mRNA synthesis | Inquire |
Biorepository
The core is equipped with a top of the line setup for cell cryopreservation and storage. Our Isothermal Liquid Nitrogen Freezers are safe; provide no cross-contamination and the best in the industry for temperature uniformity.
| Service | Description | Price |
| Vial Storage | Price per vial per month | $0.07 |
| Vial of wild‐type iPSCs | Derived from fibroblasts, reprogrammed with RNA | $760.03 |
| Vial of modified iPSCs | Constitutively fluorescent iPSCs (mWasabi; strong CAGG promoter, targeted to AAVS safe) Core generated genetically modified iPSCs | $1,287.19 |
Other Services
| Service | Description | Price |
| iPSC Training | Hands‐on training includes thawing, culture, passaging, and freezing of human iPSCs | $2,145.54 |
| Consulting | Example expertise includes but is not limited to: CRISPR design, custom assay design (eg ddPCR), viral vector design, custom mRNA production, training, etc) | $171.96/h |
Listed prices are for the researchers affiliated with the University of Colorado. External clients need to contact the core to inquire about external prices.
Click here for PDF of our full price list.