Gene Editing
The core utilizes the CRISPR/Cas9 system to achieve gene editing in iPSCs and somatic cells.
| Service | Time |
| Experimental design and reagent acquisition | |
| Targeting strategy and design | 1 week |
| gRNA and ssODN synthesis | 2-4 weeks |
| Vector synthesis (if applicable) | 6-8 weeks |
| Modify cells (mixed cell pool) | |
| Nucleofection into target cells | 2-3 weeks |
| Genome editing verification | 1-2 weeks |
| Single-cell expansion, genome editing confirmation | |
| Picking/expanding clones | 4-5 weeks |
| Colony screening (T7E1, ddPCR, etc) | 2-3 weeks |
| Sanger Sequencing | 2-3 weeks |
| iPSC clone characterization, expansion, biobanking | |
| Cell recovery, culturing, expansion | 2-4 weeks |
| Sequencing Targeted Region | 1-2 weeks |
| Pluripotency Staining | 1 week |
| Karyotyping | 2 weeks |
| Identity and mycoplasma testing | 1 week |
| Freezing | 1 week |
| Total: | 22-32 weeks |
| Add 6-8 weeks for synthesis of targeting plasmid if required |