Alert: Canvas Outage
CU is aware that Canvas is unavailable and the webpage temporarily displayed a message regarding a security breach of Canvas. The site is now displaying a maintenance message. CU is working with the vendor to investigate and resolve the issue. CU does not control the platform. We cannot provide a timeline for resolution. This incident is impacting Canvas instances globally and is part of the larger data breach reported to you on Monday. We will provide additional updates as they are available.
CU is aware that Canvas is unavailable and the webpage temporarily displayed a message regarding a security breach of Canvas. The site is now displaying a maintenance message. CU is working with the vendor to investigate and resolve the issue. CU does not control the platform. We cannot provide a timeline for resolution. This incident is impacting Canvas instances globally and is part of the larger data breach reported to you on Monday. We will provide additional updates as they are available.
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Gene Editing
The core utilizes the CRISPR/Cas9 system to achieve gene editing in iPSCs and somatic cells.
| Service | Time |
| Experimental design and reagent acquisition | |
| Targeting strategy and design | 1 week |
| gRNA and ssODN synthesis | 2-4 weeks |
| Vector synthesis (if applicable) | 6-8 weeks |
| Modify cells (mixed cell pool) | |
| Nucleofection into target cells | 2-3 weeks |
| Genome editing verification | 1-2 weeks |
| Single-cell expansion, genome editing confirmation | |
| Picking/expanding clones | 4-5 weeks |
| Colony screening (T7E1, ddPCR, etc) | 2-3 weeks |
| Sanger Sequencing | 2-3 weeks |
| iPSC clone characterization, expansion, biobanking | |
| Cell recovery, culturing, expansion | 2-4 weeks |
| Sequencing Targeted Region | 1-2 weeks |
| Pluripotency Staining | 1 week |
| Karyotyping | 2 weeks |
| Identity and mycoplasma testing | 1 week |
| Freezing | 1 week |
| Total: | 22-32 weeks |
| Add 6-8 weeks for synthesis of targeting plasmid if required |