Human Immune Monitoring Shared Resource

The Human Immune Monitoring Shared Resource (HIMSR) leveraged resources from across campus to obtain one of five IonPath’s Multiple Ion Beam Imaging (MIBI) beta units in operation worldwide. Two commercial MIBI units have now been released, and the HIMSR unit received a commercial upgrade in January 2021. The MIBI allows single cell analysis in situ using antibodies tagged with isotopically pure metal reports to image up to 100 target proteins or RNAs in fresh-frozen and FFPE tissue, with a five-log dynamic range, and 100 nanometer cellular resolution. This core provides access to cutting-edge equipment at a cost-effective price, a highly enthusiastic and skilled staff, experiment design consultation, data analysis assistance, and instrument training services to Gates Institute members.